Plasmid

Part:BBa_K112143:Design

Designed by: Madhvi Venkatesh   Group: iGEM08_UC_Berkeley   (2008-11-01)


Entry vector with OriV replication origin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 224
    Illegal XbaI site found at 2021
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 224
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 224
    Illegal BamHI site found at 1134
    Illegal XhoI site found at 3174
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 224
    Illegal XbaI site found at 2021
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 224
    Illegal XbaI site found at 2021
    Illegal NgoMIV site found at 1238
    Illegal NgoMIV site found at 1245
    Illegal NgoMIV site found at 1369
    Illegal NgoMIV site found at 3027
    Illegal AgeI site found at 872
    Illegal AgeI site found at 984
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page


Source

K112125 & pBca1256

References